27 nov. 2009 DIAGNOSTICUL COMPLEX AL LEZIUNILOR PREINVAZIVE ALE COLULUI UTERIN
D.Muresan*, Ioana Rotar*, Popp R**., Silvana Apostol*, Georgiana Coroiu***, D.Ona*, Gabriela Caracostea*, F. Stamatian*
* Clinica Ginecologie I, U.M.F. “Iuliu Hatieganu” Cluj-Napoca ** Catedra Genetica, U.M.F. “Iuliu Hatieganu” Cluj-Napoca *** Clinica de Boli Infectioase, Laboratorul de Anatomie Patologica
Rezumat
Diagnosticul si tratamentul neoplaziilor cervicale intraepiteliale reprezinta o provocare in practica ginecologica moderna. Alaturi de citologia clasica au intrat recent in practica clinica citologia monostrat si detectarea prezentei tulpinilor HPV HR prin imunohistochimie si PCR.
Pentru a compara aceste metode am realizat in Clinica Obstetrica Ginecologie I Cluj Napoca un studiu prospectiv longitudinal inrolând 52 de paciente in perioada 1 ianuarie 2008- 31 iulie 2008 cu diagnostic variind de la LSIL la carcinom microinvaziv.
Concluzia studiului a fost ca imunohistochimia este suficient de sensibila pentru practica clinica comparativ cu tehnica PCR, in conditiile unor costuri mult mai reduse. Desigur sunt necesare studii pe esantioane reprezentative pentru a confirma aceasta concluzie.
Abstract – THE COMPLEX DIAGNOSIS OF THE PREINVAZIVE CERVIX CANCER
The diagnosis and treatment of cervical intraepithelial neoplasia represents a challenge in the current gynecological practice. Beside the classical exfoliative cytology recently have entered into the clinical practice new procedures: monolayer cytology and the detection of HPV HR using immunohistochemistry and PCR.
In order the compare these new methods we have design a prospective longitudinal study. We enrolled 52 patients admitted in our hospital, the 1st Clinic of Obstetrics and Gynecology Cluj Napoca between 1st January 2008 – 31st July 2008 with diagnosis ranging from LSIL to microinvasive cancer.
Our conclusion was immunohistochemistry is sensitive enough for the clinical practice versus PCR, while the costs are lower. Future study are needed in order to support our conclusion realized on representative population
Key words: HPV HR, immunohistochemistry, PCR, intraepithelial cervical neoplasia, exfoliative cytology